Journal: Journal of Visualized Experiments : JoVE

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

doi: 10.3791/3791

Figure Lengend Snippet:

Article Snippet: However, customized antibody microarray manufacture can be readily done from a service provider such as Serome Biosciences Inc. For a robust result, we recommend a non-contact microarrayer, such as the Scienion sciFLEXARRAYER ultra low volume non-contact microarrayer which was used in our antibody microarray manufacture.

Techniques: Microarray, Staining, Incubation, Protease Inhibitor

Journal: Sensors (Basel, Switzerland)

Article Title: Multiplexed Electrochemical Detection of Yersinia Pestis and Staphylococcal Enterotoxin B using an Antibody Microarray

doi: 10.3390/s100403351

Figure Lengend Snippet: Electrochemical detection (ECD) assay for SEB. Antibody microarrays were incubated with 0 pg/mL, 1 pg/mL, 10 pg/mL, or 100 pg/mL SEB. Anti- B. anthracis , anti- Y. pestis , and anti-SEB refer to the antibodies immobilized on the chip surface. ( A ) Mean signals and SD; ( B ) Image of microarray.

Article Snippet: CombiMatrix 4X2K antibody microarrays were supplied by CombiMatrix Corp [ ].

Techniques: Incubation, Microarray

Journal: Clinical Proteomics

Article Title: Current applications of antibody microarrays

doi: 10.1186/s12014-018-9184-2

Figure Lengend Snippet: Review of planar antibody microarray technologies and their applications in the field of proteomics. Images were adopted from Servier Medical Art by Servier ( http://www.servier.com/Powerpoint-image-bank ) and modified by the authors under the following terms: CREATIVE COMMONS Attribution 3.0 Unported (CC BY 3.0)

Article Snippet: Abnova , Alzheimer’s disease , Master Antibody Microarray , 656 , 25428253.

Techniques: Microarray, Modification

Journal: Clinical Proteomics

Article Title: Current applications of antibody microarrays

doi: 10.1186/s12014-018-9184-2

Figure Lengend Snippet: Antibody microarray summary

Article Snippet: Abnova , Alzheimer’s disease , Master Antibody Microarray , 656 , 25428253.

Techniques: Microarray, Phospho-proteomics, Infection, Biomarker Discovery, Virus

Journal: Clinical Proteomics

Article Title: Current applications of antibody microarrays

doi: 10.1186/s12014-018-9184-2

Figure Lengend Snippet: Summary of commercial available antibody microarrays from different companies, Type G (Glass), M (Membrane)

Article Snippet: Abnova , Alzheimer’s disease , Master Antibody Microarray , 656 , 25428253.

Techniques: Membrane, Microarray, Biomarker Discovery, Functional Assay, Clinical Proteomics

Journal: Clinical Proteomics

Article Title: Current applications of antibody microarrays

doi: 10.1186/s12014-018-9184-2

Figure Lengend Snippet: Antibody microarray summary

Article Snippet: Medsaic Pty , Cancer , DotScanTM antibody microarrays , 144 , 27086589.

Techniques: Microarray, Phospho-proteomics, Infection, Biomarker Discovery, Virus

Journal: Frontiers in Immunology

Article Title: Applications of Protein Microarrays in Biomarker Discovery for Autoimmune Diseases

doi: 10.3389/fimmu.2021.645632

Figure Lengend Snippet: Major protein microarray platforms for biomarker discovery in autoimmune diseases.

Article Snippet: DotScanTM antibody microarray , 82 mouse monoclonal antibodies against human CD antigens , Medsaic , SLE , ( ) .

Techniques: Microarray, Biomarker Discovery, Protein Array, Recombinant, In Silico, Selection, In Situ, Peptide Microarray, Ab Array, Bioprocessing

Journal: PLoS ONE

Article Title: Mesenchymal stromal cell-derived extracellular vesicles reduce lung inflammation and damage in nonclinical acute lung injury: Implications for COVID-19

doi: 10.1371/journal.pone.0259732

Figure Lengend Snippet: (A) Antibody microarray particle analysis of tetraspanin proteins. (B) Electrochemiluminescent multiplexed immunoassay detection of tetraspanin proteins. (C) Western blot analysis of MSC-EV proteins flotillin-1, Annexin-2, Syntenin-1, MHC-I and contaminating proteins MHC-II and calreticulin. (D) Immunogold cryo-transmission electron microscopy imaging for CD63 and phosphatidylserine. Atomic force microscopy for MSC-EV morphology and size. (E) Nanoparticle tracking for MSC-EV size and concentration. (F) MSC-EV particle density correlated to phospholipid content. Error bars are SEM of the mean. *p ≤0.05 vs CD63. Scale bar denotes 100 nm.

Article Snippet: Antibody microarray bound MSC-EVs were detected at Nanoview Biosciences using reflectance imaging as previously described [ ].

Techniques: Microarray, Particle Size Analysis, Western Blot, Transmission Assay, Electron Microscopy, Imaging, Microscopy, Concentration Assay